1x zymogram renaturating buffer Search Results


1x zymogram renaturation buffer  (Bio-Rad)
93
Bio-Rad 1x zymogram renaturation buffer
1x Zymogram Renaturation Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+zymogram+renaturating+buffer/10x+Zymogram+Renaturation+Buffer/pmc03839321-119-32-36
Average 93 stars, based on 1 article reviews
1x zymogram renaturation buffer - by Bioz Stars, 2026-09
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1x renaturing buffer bio rad  (Bio-Rad)
97
Bio-Rad 1x renaturing buffer bio rad
1x Renaturing Buffer Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+zymogram+renaturating+buffer/Lysis+Buffer/pmc03174277-62-7-10
Average 97 stars, based on 1 article reviews
1x renaturing buffer bio rad - by Bioz Stars, 2026-09
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1x zymogram development buffer  (Bio-Rad)
91
Bio-Rad 1x zymogram development buffer
1x Zymogram Development Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+zymogram+renaturating+buffer/10x+Zymogram+Development+Buffer/10__3892_slash_or_00000257-38-43-47
Average 91 stars, based on 1 article reviews
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zymogram development buffer  (Bio-Rad)
94
Bio-Rad zymogram development buffer
Zymogram Development Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+zymogram+renaturating+buffer/Zymogram+Sample+Buffer/pmc03700899-96-78-81
Average 94 stars, based on 1 article reviews
zymogram development buffer - by Bioz Stars, 2026-09
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2x zymography  (Bio-Rad)
98
Bio-Rad 2x zymography
(A) Confluent cultures of porcine TM cells grown in 96-well plate were phagocytically challenged to E. coli in the presence of vehicle or DQ-gelatin (10 μg/mL), with or without Ca074Me (40 μM). Fluorescence peptides released by the enzymatic cleavage of the substrates were measured in a microplate reader at the indicated times (Em: 495 nm; Exc: 515 nm). All values were corrected for background fluorescence. Values are mean ± SD. * compare E. coli-exposed cultures versus control; # compare Ca074M-treated cultures versus non-treated, *, # p<0.05, **, ## p<0.01, ***, ### p<0.001 (t-test, n=3). (B) Confluent cultures of TM cells were subjected to phagocytic challenge to either E.coli or collagen I-coated beads for ten days. To avoid proteases contained in the serum to interfere with the assays, cells were shifted to serum-free media at day nine after phagocytic challenged. Serum-free cell culture supernatant samples (25 μl) were subjected to gelatin, casein, and plasminogen/casein gel <t>zymography.</t> Areas of proteolytic activity appeared as clear bands. Casein and plasminogen/casein color pictures have been reversed to improve sensititivity using ImageJ.
2x Zymography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+zymogram+renaturating+buffer/Tris/pmc03700899-96-13-81
Average 98 stars, based on 1 article reviews
2x zymography - by Bioz Stars, 2026-09
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1x pbtb  (Proteintech)
96
Proteintech 1x pbtb
(A) Confluent cultures of porcine TM cells grown in 96-well plate were phagocytically challenged to E. coli in the presence of vehicle or DQ-gelatin (10 μg/mL), with or without Ca074Me (40 μM). Fluorescence peptides released by the enzymatic cleavage of the substrates were measured in a microplate reader at the indicated times (Em: 495 nm; Exc: 515 nm). All values were corrected for background fluorescence. Values are mean ± SD. * compare E. coli-exposed cultures versus control; # compare Ca074M-treated cultures versus non-treated, *, # p<0.05, **, ## p<0.01, ***, ### p<0.001 (t-test, n=3). (B) Confluent cultures of TM cells were subjected to phagocytic challenge to either E.coli or collagen I-coated beads for ten days. To avoid proteases contained in the serum to interfere with the assays, cells were shifted to serum-free media at day nine after phagocytic challenged. Serum-free cell culture supernatant samples (25 μl) were subjected to gelatin, casein, and plasminogen/casein gel <t>zymography.</t> Areas of proteolytic activity appeared as clear bands. Casein and plasminogen/casein color pictures have been reversed to improve sensititivity using ImageJ.
1x Pbtb, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+zymogram+renaturating+buffer/FITC+Antibody/pm34089646-548-49-59
Average 96 stars, based on 1 article reviews
1x pbtb - by Bioz Stars, 2026-09
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ni nta agarose resin  (Qiagen)
99
Qiagen ni nta agarose resin
(A) Confluent cultures of porcine TM cells grown in 96-well plate were phagocytically challenged to E. coli in the presence of vehicle or DQ-gelatin (10 μg/mL), with or without Ca074Me (40 μM). Fluorescence peptides released by the enzymatic cleavage of the substrates were measured in a microplate reader at the indicated times (Em: 495 nm; Exc: 515 nm). All values were corrected for background fluorescence. Values are mean ± SD. * compare E. coli-exposed cultures versus control; # compare Ca074M-treated cultures versus non-treated, *, # p<0.05, **, ## p<0.01, ***, ### p<0.001 (t-test, n=3). (B) Confluent cultures of TM cells were subjected to phagocytic challenge to either E.coli or collagen I-coated beads for ten days. To avoid proteases contained in the serum to interfere with the assays, cells were shifted to serum-free media at day nine after phagocytic challenged. Serum-free cell culture supernatant samples (25 μl) were subjected to gelatin, casein, and plasminogen/casein gel <t>zymography.</t> Areas of proteolytic activity appeared as clear bands. Casein and plasminogen/casein color pictures have been reversed to improve sensititivity using ImageJ.
Ni Nta Agarose Resin, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+zymogram+renaturating+buffer/Ni-NTA+Agarose/pmc01618119-354-23-26
Average 99 stars, based on 1 article reviews
ni nta agarose resin - by Bioz Stars, 2026-09
99/100 stars
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rna tiles  (New England Biolabs)
97
New England Biolabs rna tiles
(A) Confluent cultures of porcine TM cells grown in 96-well plate were phagocytically challenged to E. coli in the presence of vehicle or DQ-gelatin (10 μg/mL), with or without Ca074Me (40 μM). Fluorescence peptides released by the enzymatic cleavage of the substrates were measured in a microplate reader at the indicated times (Em: 495 nm; Exc: 515 nm). All values were corrected for background fluorescence. Values are mean ± SD. * compare E. coli-exposed cultures versus control; # compare Ca074M-treated cultures versus non-treated, *, # p<0.05, **, ## p<0.01, ***, ### p<0.001 (t-test, n=3). (B) Confluent cultures of TM cells were subjected to phagocytic challenge to either E.coli or collagen I-coated beads for ten days. To avoid proteases contained in the serum to interfere with the assays, cells were shifted to serum-free media at day nine after phagocytic challenged. Serum-free cell culture supernatant samples (25 μl) were subjected to gelatin, casein, and plasminogen/casein gel <t>zymography.</t> Areas of proteolytic activity appeared as clear bands. Casein and plasminogen/casein color pictures have been reversed to improve sensititivity using ImageJ.
Rna Tiles, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+zymogram+renaturating+buffer/Streptavidin/pmc06523889__nanomaterials___09___00507___s001-37-16-36
Average 97 stars, based on 1 article reviews
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β tubulin monoclonal antibody  (Proteintech)
93
Proteintech β tubulin monoclonal antibody
(A) Confluent cultures of porcine TM cells grown in 96-well plate were phagocytically challenged to E. coli in the presence of vehicle or DQ-gelatin (10 μg/mL), with or without Ca074Me (40 μM). Fluorescence peptides released by the enzymatic cleavage of the substrates were measured in a microplate reader at the indicated times (Em: 495 nm; Exc: 515 nm). All values were corrected for background fluorescence. Values are mean ± SD. * compare E. coli-exposed cultures versus control; # compare Ca074M-treated cultures versus non-treated, *, # p<0.05, **, ## p<0.01, ***, ### p<0.001 (t-test, n=3). (B) Confluent cultures of TM cells were subjected to phagocytic challenge to either E.coli or collagen I-coated beads for ten days. To avoid proteases contained in the serum to interfere with the assays, cells were shifted to serum-free media at day nine after phagocytic challenged. Serum-free cell culture supernatant samples (25 μl) were subjected to gelatin, casein, and plasminogen/casein gel <t>zymography.</t> Areas of proteolytic activity appeared as clear bands. Casein and plasminogen/casein color pictures have been reversed to improve sensititivity using ImageJ.
β Tubulin Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+zymogram+renaturating+buffer/CL488-conjugated+beta+Tubulin+Antibody/pmc10028982-171-91-94
Average 93 stars, based on 1 article reviews
β tubulin monoclonal antibody - by Bioz Stars, 2026-09
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Image Search Results


(A) Confluent cultures of porcine TM cells grown in 96-well plate were phagocytically challenged to E. coli in the presence of vehicle or DQ-gelatin (10 μg/mL), with or without Ca074Me (40 μM). Fluorescence peptides released by the enzymatic cleavage of the substrates were measured in a microplate reader at the indicated times (Em: 495 nm; Exc: 515 nm). All values were corrected for background fluorescence. Values are mean ± SD. * compare E. coli-exposed cultures versus control; # compare Ca074M-treated cultures versus non-treated, *, # p<0.05, **, ## p<0.01, ***, ### p<0.001 (t-test, n=3). (B) Confluent cultures of TM cells were subjected to phagocytic challenge to either E.coli or collagen I-coated beads for ten days. To avoid proteases contained in the serum to interfere with the assays, cells were shifted to serum-free media at day nine after phagocytic challenged. Serum-free cell culture supernatant samples (25 μl) were subjected to gelatin, casein, and plasminogen/casein gel zymography. Areas of proteolytic activity appeared as clear bands. Casein and plasminogen/casein color pictures have been reversed to improve sensititivity using ImageJ.

Journal: PLoS ONE

Article Title: Cathepsin B Is Up-Regulated and Mediates Extracellular Matrix Degradation in Trabecular Meshwork Cells Following Phagocytic Challenge

doi: 10.1371/journal.pone.0068668

Figure Lengend Snippet: (A) Confluent cultures of porcine TM cells grown in 96-well plate were phagocytically challenged to E. coli in the presence of vehicle or DQ-gelatin (10 μg/mL), with or without Ca074Me (40 μM). Fluorescence peptides released by the enzymatic cleavage of the substrates were measured in a microplate reader at the indicated times (Em: 495 nm; Exc: 515 nm). All values were corrected for background fluorescence. Values are mean ± SD. * compare E. coli-exposed cultures versus control; # compare Ca074M-treated cultures versus non-treated, *, # p<0.05, **, ## p<0.01, ***, ### p<0.001 (t-test, n=3). (B) Confluent cultures of TM cells were subjected to phagocytic challenge to either E.coli or collagen I-coated beads for ten days. To avoid proteases contained in the serum to interfere with the assays, cells were shifted to serum-free media at day nine after phagocytic challenged. Serum-free cell culture supernatant samples (25 μl) were subjected to gelatin, casein, and plasminogen/casein gel zymography. Areas of proteolytic activity appeared as clear bands. Casein and plasminogen/casein color pictures have been reversed to improve sensititivity using ImageJ.

Article Snippet: Serum-free cell culture supernatant samples (25 μl) were mixed with equal volumes of 2X zymography sample buffer (125 mM Tris-HCl, pH 6.8, 50% glycerol, 8% SDS, 0.02% bromophenol blue), loaded onto SDS-PAGE gels containing gelatin, casein, or casein/plasminogen under nonreducing conditions, and electrophoresed with 2.5 mM Tris-HCl, 19.2 mM glycine, 0.01% SDS, pH 8.3, at 100 V. After electrophoresis, gels were washed with 1x renaturing buffer and 1x development buffer for 30 minutes each, and incubated overnight in zymogram development buffer (Bio-Rad).

Techniques: Fluorescence, Control, Cell Culture, Zymography, Activity Assay